Different growing places provide a difference in essential oil content that will also affect its biological action. The essential oil was obtained by hydro-distillation from the leaves of Cymbopogon nardus from Lembang West Java. Gas chromatography mass spectrometry (GC–MS) analysis revealed the presence of 53 compounds with the major constituents, including citronella (26.27%), cadinene (6, 97), methyl isoeugenol (5,87%), geranyl acetate (4,41%) and citronellyl propionate (4,97%). The antioxidant activities of the essential oils were determined using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay. The results demonstrated that essential oils have antioxidant activities with the EC50% 2.44 μg. mL−1. Finally, essential oils showed antibacterial activity against the tested Gram positive and Gram-negative bacteria. The minimum inhibitory concentration of the essential oils ranged from 250 μg. mL−1 to 1000 μg. mL−1. The essential oil content of plants grown in the highlands such as Lembang is recommended for maximum essential for antioxidant and antibacterial activity.
Keywords: Antibacterial, Antioxidant, Chemical composition, Cymbopogon nardus.