This research has studied chemical content and antimicrobial activity of methanol leaves extracts of five Ficus (Ficus ampelas, Ficus ardisioides, Ficus fistulosa, Ficus heteropoda and Ficus hirta). Antimicrobial activity was evaluated against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Candida albicans and Aspergillus Niger by using agar diffusion. The result showed that methanol leaves extracts of Ficus ardisioides and Ficus heteropoda contain flavonoid, triterpenoid and steroid compounds. Those Ficus also inhibited the growth of Sthapylococcus aureus and Bacillus subtilis with variation inhibition. The result in the present study suggested that Ficus ardisioides and Ficus heteropoda leaves can be used for treating diseases caused by Staphylococcus aureus.