Merger is combination of two or more companies to establish a new company. Merger happens when a company takes over all the operations of other business entities and the entities which taken over is dissolved. This research aims to analyze profitability of company before and after merger in companies listed in Indonesia Stock Exchange. Profitability is measured by using ratios: Net Profit Margin (NPM), Return on Equity (ROE), Return on Investment (ROI). This research takes population from all of the company that done merger in period of 2009-2014. The samples of this research consists of 3 firms from non-banking category are listed in Indonesia Stock Exchange. The ratios of data obtained from company’s financial statements are published. The analysis used to test the hypothesis of this research is descriptive and comparative analysis with a statistical test Paired Sample T Test. The results from Paired Sample T Test shows that there was difference for Net Profit Margin, Return on Equity and Return On Investment ratios before and after merger in period of observation and testing.